Generator

Part:BBa_K1021025:Design

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-13)


PtrpC+bar


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 920
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 429
    Illegal NgoMIV site found at 580
    Illegal NgoMIV site found at 826
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Fungal expression does not require an RBS so this component of a typical generator is excluded.


Source

PtrpC comes from Aspergillus nidulans; bar originates from Streptomyces hygroscopicus.

References